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dead source45974 safe2164891 artist:ankou23 applejack199659 fluttershy257400 pinkie pie254771 rainbow dash278736 rarity216712 twilight sparkle356251 earth pony442216 pegasus492575 pony1592767 unicorn533741 g42017176 animated125348 azumanga daioh100 cute264495 cute six75 daaaaaaaaaaaw6964 dashabetes12181 diapinkes12551 eyes closed138074 female1792391 flying54674 frame by frame4701 gif48416 glasses88070 group7815 happy44231 jackabetes7911 kagura13 kasuga ayumu23 mane six37468 mare734830 mihama chiyo15 mizuhara koyomi10 parody17436 pixiv14455 raribetes6991 sakaki11 sextet389 shyabetes19119 spread wings93299 sweet dreams fuel2041 takino tomo16 twiabetes15236 unicorn twilight32787 wings220252 yay956

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Background Pony #E046
@Background Pony #6BA5  
Wait, we need Gakkou Gurashi (School Live!) MLP Crossover….so far…I think  
Pinkie Pie - Yuki  
Rainbow Dash - Kurumi  
Applejack - Miki
 
I don’t know about the rest though….
Background Pony #F9F6
@Lunalove  
Then we can do with
 
Rainbow Dash: Konata Izumi  
Derpy: Tsukasa Hiragii  
Twilight Sparkle: Kagami Hirgaii  
Fluttershy: Miyuki Takara  
Applejack: Patricia Martin  
Pinkie Pie: Misao Kusakabe  
Rarity: Minami Iwasaki
 
In Lucky Star! Any more Animes we can do
Lunalove

All it needs next is:
 
Derpy: Yui (Gibson Les Paul; ‘GITAH!’)  
Fluttershy: Mugi-chan (KORG Triton Extreme keyboard)  
Rarity: Mio (Fender Jazz Bass; ‘Elizabeth’)  
Rainbow Dash: Ritsu (Yamaha drums, Zildjian cymbals)  
Twilight Sparkle: Azu-nyan (Fender Mustang guitar; ‘Muttan’)
 
Sawako-sensei: (Princess Molestia; Gibson Flying V)
 
full
 
…in “K-PON!”
 
‘Please don’t say you are Hayzy…!’
DJLowrider
The End wasn't The End - Found a new home after the great exodus of 2012

Huh. Well, I just linked it because AzuDai OP. There are other vids of it out there as well. If they actually believe that anti-vaccine bullshit, I feel sorry for them. /shrug
Background Pony #CC05
@DJLowrider  
The last ten seconds of the video are just a message saying saying the flu vaccine contains poisons. Their other videos are also Azumanga with random bits of anti-vaccine propaganda inserted for no reason, and references to an old blog (theflucase) that was apparently run by a conspiracy theorist and shut down for inciting violent anti-vaccine action.
 
It is completely inexplicable, but I think they’re serious.
KitHavoc

Pinkie - Chiyo, should be Twilight.  
Rarity - Yomi, I can see this.  
Applejack - Kagura, Rainbow Dash fits this role.  
Fluttershy - Osaka, I can see this.  
Twilight - Sakaki, Applejack would be perfect as Sakaki.  
Rainbow Dash - Tomo, hard choice but I think she should be Pinkie.
Lunalove

@lasty
 
When Chiyo-chichi is voiced by Norio Wakamoto - as is the Genetic Beast ‘TAMA’ in “Kiddy Girl-and”, a Dyson vacuum cleaner in “Haiyore! Nyaruko-san W”, and a yellow ball-thing in “Nin-Nin-Ga Shinobuden” also by him - then anything makes sense. X3
DJLowrider
The End wasn't The End - Found a new home after the great exodus of 2012

@fixman88
 
The uploader for that copy of the OP put up a “Don’t take the swine flu vaccine!” message on Yukari’s chalkboard at one point. Honestly I’ve never thought it was something to take seriously since, y’know, AzuDai. It’s the sort of dumb shit Yukari would tell her students that we’re meant to laugh at. That’s how I took it. Of course if it’s the uploader being serious then yes it’s dumb and wrong, but again I posit that the OP sequence of an incredibly silly and adorable slice-of-life anime is not where one tries to make such a point.
 
tl;dr - pretty sure it was meant as a joke, why so serious anon?