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I just rewatched that scene, and you’re right! I completely missed that.
In the ep, Screw Loose didn’t bark - as Spike was passing, Winona yipped, and the nurse pony (who had been checking in on Screw Loose but at the moment wasn’t looking right at her) gave a “Was that you?” stare at Screw Loose. Screw Loose reacted “NO NO THAT WASNT ME!” (captured perfectly here).
I think humanised Spike in his style was some kind of shapeshifter.
She barked in the episode too.
I can’t decide on whether I like him as much as clawed and be-scaled human spike though.