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commish! ^v^
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Usually unless otherwise specified, sister is assumed to mean a familial relationship, biological or adopted, so to me personally that’s how I see them.
This is a late response, but I believe Adagio refers to them as her sisters only once, in the comics.
The canonisity of the comics is debatable, but what exactly she means is also never clarified.
“Sisters” could refer to blood relation, but also adopted siblings, an expression of solidarity, or maybe members of the same coven or sorority, if that’s something sirens do.