Top one is Princess Eterna, daughter of Celestia and Discord. Has a lot on her mind, prefers to ignore it for the most part.
The ones at the bottom are the twin sisters Astral Serenity (left), and Cosmic Harmony, daughters of Flash Sentry ( hEAR ME OUT, i think he is a sweetheart, but i never really saw him with Twi) and Twilight Sparkle. Astral is very paranoid, her sister Cosmic assures her that all of the evil in Equestria is gone, that their only duty is being the heir of a peaceful land, but Astral can’t truly believe that.