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Description

MLP Special #1, re-released as  
My Little Pony n Friends: S1 E49 Escape from Midnight Castle (part 1)
 
Twilight answers little Ember’s questions about life while they relax in a meadow

Comments

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Background Pony #4726
The parental relations in G1 were rather fuzzy. I think the baby ponies were the offspring of theirs adult counterparts(in ‘bright lights’ they say something like this), since Ember isn’t called ‘Baby Twilight’ probably theirs relation is not by blood but I’m not sure.
Background Pony #CC0B
Most people seem to see their relationship that way. Of the few g1 fanfics there are, most that deal with them treat them as mother and daughter, but interestingly, I’ve never seen one where they were biologically related. Twilight is always the one who took her in after her parents died/abandoned her/something.
Amethyst_Crystal
My Little Pony - 1992 Edition
Dream Come True! - Participated in the MLP 9th Anniversary Event
A Tale For The Ages - Celebrated MLP's 35th Anniversary and FiM's 8th Anniversary
Magical Inkwell - Wrote MLP fanfiction consisting of at least around 1.5k words, and has a verified link to the platform of their choice
Birthday Cake - Celebrated MLP's 7th birthday
The End wasn't The End - Found a new home after the great exodus of 2012

Goth Hippie Nerd Pony
@FanOfMostEverything  
Unless there’s a G1 comic or package that says so, I don’t think Ember is Twilight’s daughter. All the G1 ponies were equally affectionate with each other, they were a very small community of only about 40 or so ponies.